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Transmission Electron Microscopy: TEM cryo-immunnohistochemistry of a frozen thin section of human lung using anti HT1-56 to visulize the apical surface of type 1 cells (both upper panels) and HT2-280 localized to the apical surface of type 2 cells (bottom panel).
Differential Cell Staining: Mixed alveolar epithelial cells isolated from an injured human lung specimen, cultured for 5 days on fibronectin coated plastic promoting the type 1 cell phenotype, and differentially stained with anti HT1-56 (green) and anti HT2-280 (red). Type 1 cells are stained green, attenuated type 2 cells are red, and transitional dual staining cells are yellow.
Immunohistochemistry: Human type 1 cells isolated using anti HT1-56 and cultured on 'fibronectin coated' plastic for 5 days forming a contact inhibited cell monolayer (in red), and rat type 1 cells isolated using anti RT1-40 and similarly cultured for 6 days (in green).
Our antibodies, specific within the human lung to alveolar epithelial type 1 or type 2 cells, are validated and effective for:
- Western Blotting
- Cell Isolation by FACS
- Semi-Quantitative Dot Blots
- Magnetic Cell Sorting
- TEM Cryo-Immunohistochemistry
Human lung alveolar type 2 cell labeled with antibody to HT2-280 with bound gold nanospheres. 0.1micron:
Providing validated antibodies for innovative research.
Examples of Possible Applications of Anti HT1-56 and Anti HT2-280 to
Advance Your Research.
Immunohistochemistry: Anti HT1-56 and anti HT2-280 used in conjunction to differentially illuminate alveolar type 1 cells (red) and type 2 cells (green) in a paraformaldehyde fixed thin section of a human lung specimen.
Cell Isolation by FACS: Immunofluorescence image of FACS isolated human type 1 and type 2 cells showing high purity for each differentially stained population. (A) HT1-56 positive cells (red), DAPI stained nuclei (blue). (C) HT2-280 positive cells (green). (B & D) Accompanying phase contrast images for A and C.
Western Blotting: Western Blots of whole lung homgenate run on a (A) 7% SDS-PAGE gel run under reducing conditions, transfered onto nitrocellulose, and probed using anti HT2-280, or (B) homogenate run on a 10% SDS-PAGE gel run under non-reducing conditions, and probed using anti HT1-56.
Semi-Quantitative Dot Blot: Anti HT1-56 used in a semi-quantitative dot blot to detect HT1-56 in human lung homogenate and lung aspirates from three different diseased lungs.
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