Transmission Electron Microscopy: TEM cryo-immunnohistochemistry of a frozen thin section of human lung using anti HT1-56 to visulize the apical surface of type 1 cells (both upper panels) and HT2-280 localized to the apical surface of type 2 cells (bottom panel).
Differential Cell Staining: Mixed alveolar epithelial cells isolated from an injured human lung specimen, cultured for 5 days on fibronectin coated plastic promoting the type 1 cell phenotype, and differentially stained with anti HT1-56 (green) and anti HT2-280 (red). Type 1 cells are stained green, attenuated type 2 cells are red, and transitional dual staining cells are yellow.
Immunohistochemistry: Human type 1 cells isolated using anti HT1-56 and cultured on 'fibronectin coated' plastic for 5 days forming a contact inhibited cell monolayer (in red), and rat type 1 cells isolated using anti RT1-40 and similarly cultured for 6 days (in green).
Our antibodies, specific within the human lung to alveolar epithelial type 1 or type 2 cells, are validated and effective for:
- Western Blotting
- Cell Isolation by FACS
- Semi-Quantitative Dot Blots
- Magnetic Cell Sorting
- TEM Cryo-Immunohistochemistry
Human lung alveolar type 2 cell labeled with antibody to HT2-280 with bound gold nanospheres. 0.1micron:
Providing validated antibodies for innovative research.
Examples of Possible Applications of Anti HT1-56 and Anti HT2-280 to
Advance Your Research.
Immunohistochemistry: Anti HT1-56 and anti HT2-280 used in conjunction to differentially illuminate alveolar type 1 cells (red) and type 2 cells (green) in a paraformaldehyde fixed thin section of a human lung specimen.